Research Activities
The postgraduate program at UHS aspires to higher education and research training in selected areas of Immunology. All students will carry out independent and original research under the supervision of faculty advisor.
Research Projects Completed (M.Phil Immunology- Basic Medical Sciences)
1. Association of Interleukin-4, IgE and LDL Oxidation in atherosclerosis
2. Polymorphism in methicillin resistance gene (mecA) of methicillin resistant Staphylococcus aureus isolates from Lahore and sero differences among different groups
3. Ability of different Staphlococcal aureus isolates to induce selected metabolites in human polymorphonuclaer leucocytes
4. Frequency of CD4+CD25+ T regulatory cells in males versus females
5. Levels of autoantibodies in healthy females and males
6. Enumeration of natural killer cells in diabetes mellitus patients treated with insulin and oral hypoglycemic
7. Enumeration of T regulatory cells in individuals with and without amalgam fillings in their teeth
8. Association of HLA-DR and HLA-DQ with bronchial asthma
9. Percentage of CD4+ CD25+ T regulatory cells in type 2 diabetic patients
10. Determination of serum level of IL-17 and vitamin D in asthmatic patients
11. Determination of salivary il-6 and il-17 in patients of calculus associated chronic periodontitis
12. Determination of salivary IgA ,TGF-beta, and IL-17 in patients of dental caries
13. Determination of anti endothelial cell, anti cardiolipin and anti glomerular antibodies in rheumatic heart disease patients
14. Determination of IFN-?, IL-2 and IL-10 levels in full term and preterm umbilical cord blood
15. Level of urinary CD14 in patients of acute coronary syndrome
16. Comparison of Anti cmDNA and Anti dsDNA Antibodies in Patients of Juvenile Systemic Lupus Erythematosus
17. Determination of serum NGAL level in patients of sle with and without nephritis
18. Determination of IL-10 gene polymorphism at promoter region (-592C/A and -1082A/G) and gene expression of IL-10 in patients of type 2 diabetes mellitus with and without nephropathy (Thesis Submitted)
Research Projects M.Sc MT (Completed)
1. Frequency of CD4+ and CD8+ T-lymphocytes in blood of tuberculosis patients
2. Level of interferon in the blood of tuberculosis patients
3. Determination of CRP and IL-6 in HCV positive and HCV negative patients of chronic renal failure on dialysis
4. Determination of rheumatoid factor and anti-cyclic citrulinated peptide antibodies in rheumatoid arthritis patients
5. Frequency of anti-neutrophil cytoplasmic antibody and anti-glomerular basement membrane antibody in glomerlonephritis patients
6. Detection of anti Helicobactor pylori antibodies in healthy subjects
7. Quantification of serum IgA concentration among patients of coeliac disease
8. Determination of anti-thyroid antibodies in patients of systemic lupus erythematosus
9. Prevalence of Antithyroid antibody in patients of hepatitis C infection of genotype 3a
10. Expression Analysis of Tumor Necrosis Factor Alpha (TNF-?) and Nuclear Factor Kappa Beta (NFkB) in patients of HCV genotype 3a
Research Projects Completed (M.Phil Immunology- Medical Laboratory Technology)
1. HLA DQB1 * * 0201 and DQB1 0301 alleles in patients of bronchial asthma and healthy controls visiting Gulab Devi Hospital Lahore
2. Interlukin-6 polymorphism(634C/G) in the promotor region and the progression of diabetic nephropathy in type 2 diabetes
3. Determination of Neutrophil Gelainase Associated Lipocalin (NGAL) protein in patients of systemic lupus erythematous with and without renal impairment
4. Association and polymorphism of SOCS-7 gene with interferon and insulin resistance and disease progression in chronic hepatitis C
5. Determination of Rheumatoid Factor of IgG, IgM and IgA isotypes in Rheumatoid Arthritis patients
6. Determination of antinuclear , antithyroid and anti islet cell antibodies in PCOS patients
7. Determination of TGF-B1 polymorphism at -509C/T promoter region in HCV and HCC patients
8. Polymorphism analysis of TLR 7 and TLR-9 genes in systemic lupus erythematosus
9. CD14 gene (C159T) polymorphism and its surface expression on monocytes in pulmonary TB patients
10. Determination of TLR gene polymorphism (rs4986791) and gene expression of IFN-? and TLR-4 in hepatitis C patients.
11. Determination of polymorphism +874, gene expression of IFN-? gene and serum level of IFN-? in patients of tuberculosis infections.
12. Enumeration of CD3-, CD56+, CD16+ and NKG2D+ Natural Killer Cells in Breast Cancer Patients
13. Enumeration of Circulating Tumor Cells in Breast Cancer Patients
14. Determination of anti-smooth muscles and anti-liver kidney microsomal type 1 autoantibodies and their association with HLA DR?1 (*04) allele in patients with chronic hepatitis C
15. Determination of anti-smooth muscles and anti-liver kidney microsomal type 1 autoantibodies and their association with HLA DR?1 (*04) allele in patients with chronic hepatitis C
Research Projects Ph.D (Completed)
- Role of genetics and immune mechanisms in the pathogenesis of diabetic retinopathy
- Genetic variation, expression of immune response genes and cell surface markers in Mycobacterium tuberculosis infection with and without rifampicin resistance.
Research Projects M.Phil Immunology- Basic Medical Sciences (Ongoing)
1. Determination of Polymorphism in Interferon-gamma Gene (rs+874) and levels of Interferon-gamma and Myeloperoxidase in patients with Oral Lichen Planus
2. Levels of IL-22 in diabetes mellites type 2 patients with and without nephropathy
3. Serum level of CD36 in pulmonary tuberculosis and health controls
4. Surface expression of CD40Ligand on T-cell and its level in pulmonary tuberculosis patients with or without treatment
5. Determination of anti-Pentraxin 3 and anti-dsDNA levels in patients of SLE with and without proteinuria and in healthy conrols.
6. Levels of Anti-Apolipoprotein A 1 autoantibodies in patients of Rheumatoid Arthritis and health control
Research Projects M.Phil Immunology- Medical Laboratory Technology (Ongoing)
- Determination of PD-L1 gene polymorphism (A/L 8923) and gene expression analysis in oropharyngeal squamous cell carcinoma
. Determination of Th17 cells in TB patients
Research Projects Ph.D (Ongoing)
. HLA profile and immune markers in methotrexate responders and non-responders juvenile idiopathic arthritis patients
. Genetic variation and expression analysis of GIMAP4, IFN-? and interlukin-17 in patients of Tuberculosis with and without Drug resistance
. Identification and Characterization of Cancer Stem Cells in Head & Neck Squamous Cell Carcinoma with and without Human PapillomaVirus.
. Whole Genome Sequencing expression and gene variation analysis of SARS-CoV-2 and host genes(ACE-2, TLR, 2 and4) and immunological pattern in COVID-19 local patients.
HEC funded projects
1. Role of genetics and immune mechanisms in the pathogenesis of diabetic retinopathy. (HEC)
2. Gene expression analysis of cellular genes related to HCV induced pathogenesis. (HEC)
3. Inflammatory Cytokines, Allergy and Atherosclerosis
- Levels of autoantibodies in healthy females and males
5. Immunoglobulin light chain allelic inclusion in systemic lupus erythematosus and Rheumatoid Arthritis
6. Immunological, Viral and Genetic basis of COVID-19 in local patients (Rapid Research Grant) Word Bank
7. Molecular Characterization of Colistin and KPC bacterial resistance isolated from Hospitals and poultry industry & invitro efficacy of Silver Nano particles against MDR. (HEC/NRPU)
8. Molecular characterization of (NDM) producing microorganisms and efficacy of Manuka honey against NDM positive strains�. (HEC)/NRPU
9. Molecular and clinical characterization of cancer in Pakistan (Co-PI). (Thematic Project) .
Student Repository
Year |
Name of Student |
Programme |
Research Topic |
Supervisor |
Co-Supervisor |
|
1 |
2005 |
Dr. Farhana Shazad |
M. Phil Basic |
Association of Interleukin-4, IgE and LDL Oxidation in atherosclerosis |
Dr. Nadeem Afzal |
|
2 |
2006 |
Dr. Gul Afshan |
M. Phil Basic |
Frequency of CD4+CD25+ T regulatory cells in males versus females |
Dr. Abdul Ghaffar |
|
3 |
Numan Javed |
M. Phil Basic |
Ability of different Staphlococcal aureus isolates to induce selected metabolites in human polymorphonuclaer leucocytes |
Dr. Nadeem Afzal |
||
4 |
Shahid Hussain |
M. Sc |
Level of interferon in the blood of tuberculosis patients |
Dr. Nadeem Afzal |
||
5 |
Khursheed Javaid |
M. Sc |
CD4+ and CD8+ T-lymphocytes in blood of tuberculosis patients |
Dr. Nadeem Afzal |
||
6 |
2007 |
Romeeza Tahir |
M. Phil Basic |
Polymorphism in methicillin resistance gene (mecA) of methicillin resistant Staphylococcus aureus isolates from Lahore and sero differences among different groups |
Dr. Nadeem Afzal |
|
7 |
Sarwar Abbas |
M. Sc |
Determination of CRP and IL-6 in HCV positive and HCV negative patients of chronic renal failure on dialysis |
Dr. Nadeem Afzal |
||
8 |
Sarah Kareem |
M. Sc |
Determination of rheumatoid factor and anti-cyclic citrulinated peptide antibodies in rheumatoid arthritis patients |
Dr. Nadeem Afzal |
||
9 |
Maria Arif |
M. Sc |
Frequency of anti-neutrophil cytoplasmic antibody and anti-glomerular basement membrane antibody in glomerlonephritis patients |
Dr. Nadeem Afzal |
||
10 |
2008 |
Amna Habib |
M. Sc |
Quantification of serum IgA concentration among patients of coeliac disease |
Dr. Nadeem Afzal |
|
11 |
Mehreen Ahmad |
M. Sc |
Detection of anti Helicobactor pylori antibodies in healthy subjects |
Dr. Nadeem Afzal |
||
12 |
2009 |
MuqaddasRiaz |
M. Sc |
Determination of anti-thyroid antibodies in patients of systemic lupus erythematosus |
Dr. Nadeem Afzal |
|
13 |
2010 |
Sana Tumori |
M. Sc |
Prevalence of Antithyroid antibody in patients of HCV of genotype 3a |
Dr. Nadeem Afzal |
|
14 |
Fatima Shabir |
M. Sc |
Expression Analysis of Tumor Necrosis Factor Alpha (TNF-?) and Nuclear Factor Kappa Beta (NFkB) in patients of HCV genotype 3a |
Dr. Shah Jahan |
||
15 |
2011 |
Faheem Shahzad |
M. Phil Basic |
Percentage of CD4+ CD25+ T regulatory cells in type 2 diabetic patients |
Dr. Nadeem Afzal |
|
16 |
Foqia Naz |
M. Phil Basic |
Determination of serum level of IL-17 and vitamin D in asthmatic patients |
Dr. Nadeem Afzal |
||
17 |
2012 |
Dr. Sarah Ashraf |
M. Phil Basic |
Levels of autoantibodies in healthy females and males |
Dr. Nadeem Afzal |
|
18 |
Dr. Rabia Tufail |
M. Phil Basic |
Enumeration of natural killer cells in diabetes mellitus patients treated with insulin and oral hypoglycemic |
Dr. Nadeem Afzal |
||
19 |
Dr. Zarghoona Jaffar |
M. Phil Basic |
Determination of IFN-g, IL-2 and IL-10 levels in full term and preterm umbilical cord blood |
Dr. Nadeem Afzal |
||
20 |
Dr. Fahad Khaliq |
M. Phil Basic |
Enumeration of T regulatory cells in individuals with and without amalgam fillings in their teeth |
Dr. Nadeem Afzal |
||
21 |
Khursheed Javaid |
M. Phil Basic |
Association of HLA-DR and HLA-DQ with bronchial asthma |
Dr. Nadeem Afza |
||
22 |
2013 |
Bushra Mubark |
M. Phil MLS |
HLA DQB1 * * 0201 and DQB1 0301 alleles in patients of bronchial asthma and healthy controls visiting Gulab Devi Hospital Lahore |
Dr. Nadeem Afzal |
|
23 |
Rakhshanda Talib |
M. Phil MLS |
Interlukin-6 polymorphism(634C/G) in the promotor region and the progression of diabetic nephropathy in type 2 diabetes |
Dr. Nadeem Afzal |
Dr. Saba Khaliq |
|
24 |
Sabrina Rashid |
M. Phil MLS |
Determination of Neutrophil Gelainase Associated Lipocalin (NGAL) protein in patients of systemic lupus erythematous with and without renal impairment |
Dr. Nadeem Afzal |
||
25 |
Rabia Aslam |
M. Phil MLS |
Association and polymorphism of SOCS-7 gene with interferon and insulin resistance and disease progression in chronic hepatitis C |
Dr. Nadeem Afzal |
Dr. Saba Khaliq |
|
26 |
Sana Zainab |
M. Phil MLS |
Determination of Rheumatoid Factor of IgG, IgM and IgA isotypes in Rheumatoid Arthritis patients |
Dr. Nadeem Afzal |
||
27 |
2014 |
Hifzah Mobeen |
M. Phil MLS |
Determination of antinuclear , antithyroid and anti islet cell antibodies in PCOS patients |
||
28 |
Almina Ramiz |
M. Phil MLS |
Determination of TGF-B1 polymorphism at -509C/T promoter region in HCV and HCC patients |
Dr. Shah Jahn |
Dr. Nadeem Afzal |
|
29 |
Adil Basir |
M. Phil MLS |
Polymorphism analysis of TLR 7 and TLR-9 genes in systemic lupus erythematosus |
Dr. Shah Jahn |
Dr. Nadeem Afzal |
|
30 |
D. Hussiah Batool |
M. Phil Basic |
Determination of salivary il-6 and il-17 in patients of calculus associated chronic periodontitis |
Dr. Nadeem Afzal |
||
31 |
Dr. Ghanyia Ali |
M. Phil Basic |
Determination of serum NGAL level in patients of sle with and without nephritis |
Dr. Nadeem Afzal |
||
32 |
Dr. Ambreen Nawaz |
M. Phil Basic |
Determination of salivary IgA ,TGF-beta, and IL-17 in patients of dental caries |
Dr. Nadeem Afzal |
||
33 |
Dr. Aqsa Wajid |
M. Phil Basic |
Determination of anti endothelial cell, anti cardiolipin and anti glomerular antibodies in rheumatic heart disease patients |
Dr. Nadeem Afzal |
||
34 |
2015 |
Naila Irum |
M. Phil MLS |
Determination of TLR gene polymorphism (rs4986791) and gene expression of IFN-? and TLR-4 in hepatitis C patients. |
Dr. Shah Jahan |
Dr. Nadeem Afzal |
35 |
Neelam Majeed |
M. Phil MLS |
Enumeration of CD3-, CD56+, CD16+ and NKG2D+ Natural Killer Cells in Breast Cancer Patients |
Dr. Nadeem Afzal |
Dr. Samina Khokher |
|
36 |
Marwah Minhas |
M. Phil MLS |
Enumeration of Circulating Tumor Cells in Breast Cancer Patients |
Dr. Nadeem Afzal |
Dr. Samina Khokher |
|
37 |
Afzal Afzal |
M. Phil MLS |
Determination of polymorphism +874, gene expression of IFN-? gene and serum level of IFN-? in patients of tuberculosis infections. |
Dr. Shah Jahan |
Dr. Nadeem Afzal |
|
38 |
Dr. Wardah Irshad |
M. Phil Basic |
Level of urinary CD14 in patients of acute coronary syndrome. |
Dr. Nadeem Afzal |
||
39 |
Dr. Saima Pervaiz |
M. Phil Basic |
Comparison of Anti cmDNA and Anti dsDNA Antibodies in Patients of Juvenile Systemic Lupus Erythematosus |
Dr. Nadeem Afzal |
Dr. Asma Mushtaq |
|
40 |
2016 |
Atia Ali |
M. Phil MLS |
CD14 gene (C159T) polymorphism and its surface expression on monocytes in pulmonary TB patients |
Dr. Nadeem Afzal |
Dr. Romeeza Tahir |
41 |
Amna Pervaiz |
M. Phil MLS |
Determination of PD-L1 gene polymorphism (A/L 8923) and gene expression analysis in oropharyngeal squamous cell carcinoma |
Dr. Shah Jahan |
Dr. Nadeem Afzal |
|
42 |
2017 |
Fatima Ayub |
M. Phil MLS |
Determination of anti-smooth muscles and anti-liver kidney microsomal type 1 autoantibodies and their association with HLA DR?1 (*04) allele in patients with chronic hepatitis C |
Dr. Nadeem Afzal |
Dr. Romeeza Tahir |
43 |
2018 |
Dr. Attia Latif |
M. Phil Basic |
Determination of IL-10 gene polymorphism at promoter region (-592C/A and -1082A/G) and gene expression of IL-10 in patients of type 2 diabetes mellitus with and without nephropathy |
Dr. Shah Jahan |
Dr. Nadeem Afzal |
44 |
Dr. Ghanwa Saeed |
M. Phil Basic |
Determination of Polymorphism in Interferon-gamma Gene (rs+874) and levels of Interferon-gamma and Myeloperoxidase in patients with Oral Lichen Planus |
Dr. Romeeza Tahir |
Dr. Nadeem Afzal |
|
45 |
Dr. Maryam Aziz |
M. Phil Basic |
Levels of IL-22 in diabetes mellites type 2 patients with and without nephropathy |
Dr. Nadeem Afzal |
Khursheed Javaid |
|
46 |
Dr. Sana Mahmood |
M. Phil Basic |
Serum level of CD36 in pulmonary tuberculosis and health controls |
Nadeem Afzal |
Dr. Faheem Shahzad |
|
47 |
Dr. Sarah Samar |
M. Phil Basic |
Surface expression of CD40Ligand on T-cell and its level in pulmonary tuberculosis patients with or without treatment |
Dr. Nadeem Afzal |
Khurshee Javaid |
|
48 |
Ms. Shagufta Jabeen |
M. Phil MLS |
Determination of Th17 cells in TB patients |
Dr. Nadeem |
Dr. Romeeza Tahir |
|
49 |
2019 |
Dr. Maria Noor |
M. Phil Basic |
Determination of anti-Pentraxin 3 and anti-dsDNA levels in patients of SLE with and without proteinuria and in healthy conrols. |
Dr. Nadeem |
Khursheed Javaid |
50 |
Dr. Aisha Kiran |
M. Phil Basic |
Levels of Anti-Apolipoprotein A 1 autoantibodies in patients of Rheumatoid Arthritis and health control |
Dr. Romeeza Tahir |
Prof. Dr. Nadeem Afzal Dr. M. Haroon |
|
51 |
Alam Khan |
M. Phil MLS |
Doing part-1 |
|||
52 |
2020 |
Faiza Ilyas |
M. Phil MLS |
Doing part-1 |
||
53 |
Sasiha-ul-Ain |
M. Phil MLS |
||||
54 |
Rabia Saddiqua |
M. Phil MLS |
||||
55 |
Muhammad Asif |
M. Phil MLS |
||||
56 |
Nimra Shahzadi |
M. Phil Basic |
||||
57 |
Aafshar |
M. Phil Basic |
Ph. D Students
S# |
Year |
Name |
Topic |
Supervisor |
Co-supervisor |
||
1 |
2012 |
Dr. Nadeem Afzal |
Role of genetics and immune mechanisms in the pathogenesis of diabetic retinopathy |
Dr. A.H. Nagi |
|||
2 |
2013 |
Dr. Farhana Shahzad |
HLA profile and immune markers in methotrexate responders and non-responders juvenile idiopathic arthritis patients |
Dr. Nadeem Afzal |
Dr. Romeeza Tahir |
||
3 |
2015 |
Faheem Shahzad |
Genetic variation, expression of immune response genes and cell surface markers in Mycobacterium tuberculosis infection with and without rifampicin resistance |
Dr. Nadeem Afzal |
Dr. Shah Jahan |
||
4 |
Dr. Muhammad Kashif |
Identification and Characterization of Cancer Stem Cells in Head & Neck Squamous Cell Carcinoma with and without Human Papilloma Virus |
Dr. Nadeem Afzal |
Dr. Saima Ch |
|||
5 |
2017 |
Khursheed Javaid |
Genetic variation and expression analysis of GIMAP4, IFN-? and interlukin-17 in patients of Tuberculosis with and without Drug resistance |
Dr. Nadeem Afzal |
Dr. Romeeza Tahir |
||
6 |
2019 |
Alminah Shafiq |
Whole Genome Sequencing expression and gene variation analysis of SARS-CoV-2 and host genes(ACE-2, TLR, 2 and4) and immunological pattern in COVID-19 local patients |
Dr. Shah Jahan |
Dr. Nadeem afzal |
||
7 |
Wardah Irshad |
Literature Searching and synopsis writing |
Dr. Romeeza tahir |
||||
